mab377 ab 2298772 mouse anti coup tf2 nr2f2 coup tf ii nr2f2  (R&D Systems)

Journal: ACS Omega

Article Title: SNAP-Tag-Based Antibody–Drug Conjugates Targeting Epidermal Growth Factor Receptor 1, Epidermal Growth Factor Receptor 2, Trophoblast Cell-Surface Antigen 2, and Tissue Factor for Ovarian Cancer Treatment

doi: 10.1021/acsomega.5c10377

Figure Lengend Snippet: Different expression in ovarian cancer cell lines. The expression levels of EGFR, Trop2, TF, and Her2 in ovarian cancer cell lines were determined by flow cytometry. The cells were treated with anti-EGFR (EGFR monoclonal antibody, H11), anti-Trop2 (Trop2 monoclonal antibody, MR54), anti-TF (CD142 antibody), and anti-Her2 (ErbB2 monoclonal antibody, 3B5) antibodies, respectively, followed by incubation with a secondary antibody (Goat anti-Mouse IgG Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647). The cells were permeabilized to determine the Her2 expression according to manufacturer’s instructions.

Article Snippet: Briefly, 4 × 10 5 cells were collected and washed twice with PBS and then stained with anti-EGFR (H11, 0.5 μg, Thermo Fisher Scientific), anti-Trop2 (MR54, 1 μg, Thermo Fisher Scientific), or anti-TF (CD142, 10 μL, Miltenyi Biotec) antibodies in 200 μL of PBS for 30 min on ice.

Techniques: Expressing, Flow Cytometry, Incubation

Journal: STAR Protocols

Article Title: Protocol for mapping murine transcription factor interactomes and composite motifs combining affinity purification mass spectrometry and ChIP-seq

doi: 10.1016/j.xpro.2025.104184

Figure Lengend Snippet: Generation of the IRF4 Bio mouse strain and in vitro differentiation of primary T cells into Th17 cells (A) Schematic overview of the bacterial artificial chromosome (BAC) transgene production for the generation of the IRF4 Bio mouse strain. (B) Cross-breeding of animals: IRF4 Avi mice, containing the BAC transgene, are crossed with ROSA26 BirA mice, ubiquitously expressing BirA ligase under the ROSA26 promoter, resulting in offsprings that express in vivo biotinylated IRF4 (IRF4 Bio ). (C) Western blot analyses of full cellular lysates and nuclear extracts demonstrate successful in vivo biotinylation of IRF4 (left, anti-IRF4 antibody; right, horseradish peroxidase-conjugated streptavidin [SA-HPO]). (D) Workflow for in vitro differentiation of primary Th17 cells.

Article Snippet: For example, in the case of the TF IRF4, good results were achieved using the μMACS FactorFinder Kit by Miltenyi, which has been discontinued.

Techniques: In Vitro, Expressing, In Vivo, Western Blot

Journal: STAR Protocols

Article Title: Protocol for mapping murine transcription factor interactomes and composite motifs combining affinity purification mass spectrometry and ChIP-seq

doi: 10.1016/j.xpro.2025.104184

Figure Lengend Snippet: Integrated analysis of IRF4 interactors and double-motif occurrence (A) Double-motif occurrence of IRF4 interactors in Th17 ChIP-seq peaks. Heatmap shows normalized co-occurrence frequencies (0–1) for IRF4 together with transcription factors detected by AP-MS, considering only motifs occurring within <5 bp spacing in the same peak. Asterisk (∗): GTF2IRD1-isoform2. (B) Volcano plot of IRF4 interactors identified by AP-MS comparing biotinylated IRF4 (“Bio”) and control (“Ctrl”) conditions in Th17 cells. Labeled interactors indicate TFs with double-motif occurrences in Th17. (C) Sequence logos of the individual TF motifs used for double-motif annotation. (D) STRING-based IRF4 interaction network displaying interactors classified as TFs according to the criteria by Lambert et al. (STRING DB default settings). See also .

Article Snippet: For example, in the case of the TF IRF4, good results were achieved using the μMACS FactorFinder Kit by Miltenyi, which has been discontinued.

Techniques: ChIP-sequencing, Protein-Protein interactions, Control, Labeling, Sequencing